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KMID : 0381120220440040425
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Genes and Genomics
2022 Volume.44 No. 4 p.425 ~ p.433
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Characteristics of circRNAs expression profiles in the piglets intestine induced by oxidative stress
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Li Zhi-Xin
Chen Wei
Qin Ming
Wang Li-Xue
Zeng Yong-Qing
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Abstract
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Backgroud: Oxidative stress (OS) can affect the expression of key genes and destroy the intestinal structure. However, it is unclear how OS regulates the expression of circular RNAs (circRNAs), microRNAs (miRNAs) and mRNAs.
Objective: The aim of this study was to examine the expression of circRNAs, miRNAs and mRNAs exposed to OS.
Methods: Piglets were exposed to diquat (DQ), a herbicide, and the activity of antioxidant enzymes and the morphology of the intestine were investigated. We utilized whole transcriptome sequencing to examine the global expression of circRNAs, miRNAs and mRNAs in the jejunum.
Results: Compared to controls, 751 circRNAs, 731 miRNAs and 164 mRNAs were differentially expressed in diquat-treated piglets. Kyoto Encyclopedia of Genes and Genomes pathway analysis indicated that oxidative phosphorylation, RNA degradation and ubiquitin-mediated proteolysis were closely associated with OS.
Conclusions: Our results indicated that diquat-induced OS alters the intestinal structure, resulting in the differential expression of circRNAs, miRNAs and mRNAs in the jejunum of piglets. Meanwhile, OS weakened the enzyme antioxidant system in serum of piglets. Our results provide a foundation for further studies on the mechanisms involved in the response to OS in the jejunum.
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KEYWORD
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Oxidative stress, Non-coding RNA, Transcriptome, Jejunum, Piglets
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